Table 1.

RT-PCR Primers for TRP channels in BBB endothelial cells.

	Forward primer	Reverse primer	Amplicon size (bp)
TRPC1	gta ccc gag cac gga cct	aac agc att tct ccc aag ca	250
TRPC2	ttg ctg gga gag tct ctg gt	tca atc atc ttg cca atg ga	452
TRPC3	att ctt cga agc ccc ttc at	ctc ctt gca ctc aga cca ca	234
TRPC4	gct gga gga gaa gac cat gg	gac ctg tcg atg tgc tga ga	211
TRPC5	taa cct gca tgc cca ttg ga	ccc ttg gac gag aac cat ta	405
TRPC6	caa tcg cgg tgg ttt taa gt	cgc atc atc ctc aat ttc ct	416
TRPC7	cac gac atc aca ccc atc at	gag gac agg gtc ttc act gg	234
TRPV1	tga cta ccg gtg gtg ttt ca	tga tcc ctg cat agt gtc ca	250
TRPV2	tga tga agg ctg tgc tga ac	cag agc atg cag gac tgt gt	452
TRPV3	ctg gcc aag gaa gaa cag ag	gcc tct tcc gtg tac tca gc	234
TRPV4	atc aac tcg ccc ttc aga ga	ggt gtt ctc tcg ggt gtt gt	211
TRPV5	act gca tgt agc tgc cct ct	aca ggc aaa ggt ttt gtt gg	405
TRPV6	gca ctg ttc agc acc ttt ga	gac cat act ctc gcc cac at	416
TRPM1	gct tcg aag aca gct gga aa	cgc agg gac tcg ttt atg ac	246
TRPM2	cag agc aaa cga agg aaa gg	tca gta acg cct gca gaa tg	387
TRPM3	tgg gcc gaa aaa tct atg ag	gat cat tcc cac cga gaa ga	299
TRPM4	gag agg atc atg acc cga aa	gtc att cag cag agc atc ca	255
TRPM5	atg tcc tgc tgg tgg act tc	agc cat acg ctc agg aag aa	400
TRPM6	tga cca gtt gaa tgc aga gc	gag gct ctt gag ggc ttt tt	302
TRPM7	gct cca tgg gga gtg ata ga	atc aaa gcc acc aca gga ac	254
TRPM8	ttc tgg tca acc tcc tgg tc	taa acc gat gcc tca tct cc	339

All primers are listed in 5′-3′ orientation. The size of the expected amplified fragment is included. For details on the RT-PCR procedure, see Materials and Methods.