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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Immunogenetics. 2010 Sep 30;62(11-12):773–780. doi: 10.1007/s00251-010-0481-9

Figure 3. MCM MHC class I haplotypes.

Figure 3

MHC class I A, B, and I transcripts associated with each MCM haplotype were identified by pyrosequencing of three overlapping cDNA-PCR amplicons. Our cohort for this experiment included ten animals, seven homozygotes and three heterozygotes. The transcripts that were not previously detected by cloning and Sanger sequencing are denoted in bold. Further, the Mafa-B transcripts are divided into major and minor transcripts. We defined minor transcripts as those present at less than 1% of the total MHC class I reads identified in total PBMC. Two putative minor transcripts, denoted by a +, were identified using the 190-bp amplicon in Wiseman et al. 2009 but not in this experiment.