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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 24;79(6):1515–1528. doi: 10.1111/j.1365-2958.2010.07536.x

Fig. 3.

Fig. 3

Bacterial 2 hybrid analysis of MinD mutants that fail to respond to MinE but activate MinC. MinD and MinE were fused to the C-termini of the T18 and T25 fragments of adenyl cyclase. Plasmids containing these fusions were cotransformed into BTH101 (Δmin). Three transformants for each were picked into LB and spotted on LB plates supplemented with X-gal and antibiotics. The appearance of blue color indicates a positive reaction. The positive control (containing bZIP in both vectors) and the negative control (plasmids expressing T25-MinD and the T18 empty vector) were included for reference. The plates were incubated overnight at 30°C.