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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 24;79(6):1515–1528. doi: 10.1111/j.1365-2958.2010.07536.x

Fig. 6.

Fig. 6

Example of tests of mutants obtained by site-directed mutagenesis of minD. Spot tests were used to determine if MinD mutants activated MinC (no growth in the absence of MinE). Mutants, such as MinD-D93R activated MinC and responded to MinE, whereas MinD-K94D did not activate MinC. To see if MinD-K94D interacts with MinE it was tested in the bacterial 2-hybrid system (minD alleles are shown to the right of the test strip). If, like minD-K94D, they did not interact with minE, they were tested for self-interaction (minD-K94D and minD-V161E did self-interact whereas minD-D159R did not). Results for all mutants are summarized in Table 1 of the supporting information.