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. 2010 Dec 20;50(5):838–851. doi: 10.1093/rheumatology/keq380

Fig. 1.

Fig. 1

Expression of IL-6 and IL-8 in AGE-BSA-stimulated primary human OA chondrocytes. Effect of AGE-BSA on the gene expression of IL-6 (a) and IL-8 (b) in primary human OA chondrocytes. Primary human OA chondrocytes were treated with AGE-BSA (5–100 µg/ml) and native BSA (100 µg/ml) for 24 h. Time-dependent effect of AGE-BSA on the gene expression of IL-6 (c) and IL-8 (d) in human OA chondrocytes. Primary chondrocytes were treated with AGE-BSA (100 µg/ml) and native BSA (100 µg/ml) for 0–24 h. Expression of IL-6 or IL-8 mRNA was determined by real-time qRT–PCR using comparative ΔΔCT method. Incubation with native BSA (nBSA) was used as control. Results are representative [mean (s.e.m.)] of duplicate experiments with OA chondrocytes obtained from five age- and sex-matched OA donors and differ without a common letter; P < 0.05.