Figure 4. Roscovitine rescues the cellular phenotypes of TS cardiomyocytes.

a) Spontaneous Ca²⁺ transients in TS CMs before (black) and after treatment with 33.3 μM Ros (red) as well as after wash out (blue). Arrowheads show irregular Ca²⁺ elevations. b) Effects of Ros on the relative irregularity of the amplitude and timing of the spontaneous Ca²⁺ transients in TS CMs (n=8 cells in 2 lines, *P<0.05, **P<0.01). c) Ba²⁺ currents in TS CMs recorded in voltage-clamp mode before (black) during (red) and after (blue) treatment with 33.3 μM Ros. Ros promoted inactivation of currents in TS CMs. d) Effects of Ros on CaV1.2 VDI in CMs (n=5 cells in 2 lines, **P<0.01). e) Spontaneous APs recorded in current-clamp recording before, during and after treatment with Ros. Arrowheads show putative DADs. f) Ros prevented AP prolongation observed in TS CMs (n=8 cells in 2 lines, **P<0.01, mean ± s.e.m.).