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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Oral Microbiol. 2011 Jan 27;26(2):150–163. doi: 10.1111/j.2041-1014.2010.00600.x

Table 2.

Gene expression profile in KB cells induced by Fusobacterium nucleatum and/or Streptococcus cristatus, as determined by cytokine arrays1

Gene2 Description Expression ratio3 Fn/Fn+Sc ratio4
Fn Sc Fn+Sc
IL1α Interleukin 1, alpha 9.92 1.41 2.53 3.92
IL6 Interleukin 6 1.16 0.65 0.38 3.08
IL18 Interleukin 18 0.77 0.44 0.40 1.90
CCL16 Chemokine ligand 16 3.84 1.31 2.65 1.45
LTB Lymphotoxin beta 3.56 1.61 2.51 1.41
TNFα Tumor necrosis factor, 2.32 0.85 1.64 1.41
XCL2 Chemokine ligand 2 2.56 1.06 1.84 1.40
CCL19 Chemokine ligand 19 2.44 1.28 1.84 1.32
IL11 Interleukin 11 2.17 1.21 1.65 1.31
CXCL11 Chemokine ligand 11 2.31 0.92 1.77 1.30
CCL2 Chemokine ligand 2 2.50 1.31 2.11 1.19
CCL13 Chemokine ligand 13 2.68 1.49 2.32 1.15
CCL22 Chemokine ligand 22 2.93 1.89 2.84 1.03
CCL15 Chemokine ligand 15 2.12 1.48 2.08 1.02
CCL7 Chemokine ligand 7 1.93 1.35 2.06 0.94
IL10Rα Interleukin 10 receptor, alpha 4.82 2.06 6.64 0.73
CCR9 Chemokine receptor 9 1.47 1.59 2.04 0.72
CCL1 Chemokine ligand 1 1.50 2.79 2.52 0.60
IL4 Interleukin 4 2.75 4.89 5.86 0.47
Overall mean5 2.83 ± 0.86 1.56 ± 0.97 2.40 ± 1.46
1

After background subtraction, the signal intensity of each gene present was normalized to that of GAPDH. The expression levels of different genes after bacterial exposure were compared with those from uninfected controls and expressed as relative ratios. Only the genes that had a fold change > 2-fold or < 0.5-fold in any condition (shown in bold) were included for analysis.

2

Genes whose expression levels were significantly modulated by any experimental treatment. They were sorted in a descending order according to the Fn/Fn+Sc ratio.

3

Values represent mean relative ratios from two independent experiments.

4

Fold changes in expression levels when comparing F. nucleatum and F. nucleatum plus S. cristatus. Fn, F. nucleatum; Sc, S. cristatus; Fn+Sc, F. nucleatum plus S. cristatus.

5

Values represent the mean ± SD for each ratio column. Statistical comparison showed that the overall expression level induced by S. cristatus was significantly lower than F. nucleatum (P = 0.0046), as well as lower than F. nucleatum plus S. cristatus (P = 0.04). However, the difference between F. nucleatum and F. nucleatum plus S. cristatus was not significant.