Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Cancer Res. 2011 Jan 12;9(2):133–148. doi: 10.1158/1541-7786.MCR-10-0394

Figure 1.

Figure 1

A. RAGE and mAbGB3.1 glycans are expressed on colon tumor cells. MC38 colon tumor cells in culture were analyzed for surface expression of mAbGB3.1 glycans and RAGE by flow cytometry. Cells were stained with mAbGB3.1 or anti-RAGE followed by FITC-conjugated anti-mouse or anti-rabbit Ig. Unstained cells (filled) and cells stained with secondary antibody alone (dark line) served as negative control. B and C. Receptor on colon tumor cells for S100A8/A9 identified by co-immunoprecipitation. MC38 cells were incubated with purified mouse S100A8/A9, S100A8 or S100A9 and bound proteins were immunoprecipitated with anti-S100A8 and/or anti-S100A9, or an irrelevant control rabbit IgG. To confirm potential interaction of RAGE with endogenous S100 proteins, immunoprecipitation was also performed using MC38 cells in which endogenous S100A9 was silenced using target-specific siRNA. Whole cell lysates and immunoprecipitated proteins were separated on SDS-PAGE gels, transferred and immunoblotted with anti-RAGE (B) or anti-TLR4 (C).