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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Cancer Res. 2011 Jan 12;9(2):133–148. doi: 10.1158/1541-7786.MCR-10-0394

Figure 3.

Figure 3

S100A8/A9 activates NF-κB in colon tumor cells. A. MC38 or Caco-2 cells were incubated with purified endotoxin-free mouse or human S100A8/A9 for different periods of time, and cell lysates were analyzed by Western blot using respective antibodies against phosphorylated or total IκBα or β–actin. B. MC38 or Caco-2 cells were treated with respective purified S100A8/A9 for 6 h in the presence or absence of mAbGB3.1 or anti-RAGE and nuclear extracts were analyzed for NF-κBp65 using DNA-binding ELISA.