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. 2011 Apr 15;22(8):1389–1397. doi: 10.1091/mbc.E10-10-0827

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© 2011 Huang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Two isoforms of UXT localize in nucleus and cytoplasm, respectively. (A) Sequence alignment of UXT-V1 against UXT-V2. (B) RT-PCR analysis of UXT mRNA in HeLa cells. (C) Immunoblot of UXT in several cell lines. (D) Immunoblot of UXT in UXT-RNAi HeLa cells or controls. (E) Subcellular fractionation analysis in HeLa cells. Control antibodies indicate accuracy of fractionation (Sp1, nucleus; α-tubulin, cytoplasm). (F) Confocal microscopy analysis of HeLa cells transfected with UXT-V1 or UXT-V2, with EGFP tagged at the N terminus. Scale bar: 10 μm.