FIGURE 4:

TRAF2-binding motif is critical for UXT-V1’s anti-apoptosis function. (A) The mutation sites of UXT-V1(2M) in reference to UXT-V1(WT). (B) HEK293T cells were transfected with indicated combinations of plasmids. Cell lysates were immunoprecipitated with anti-Flag antibody, then probed by anti-HA and anti-Flag antibodies, respectively. (C and D) HeLa cells were transfected with UXT-V1 siRNA, then rescued with UXT-V1(WT), UXT-V1(2M), or UXT-V2 expression plasmids, respectively, followed by PI staining analysis and Western blot analysis. WT, wild type. * indicates nonspecific band. Data in (D) are shown as mean + SD of three independent experiments.