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. 2011 Apr 15;22(8):1148–1166. doi: 10.1091/mbc.E10-08-0722

FIGURE 2:

FIGURE 2:

Pattern of expression of Arf6-EGFP constructs and endogenous MHC-I molecules on permissive lymphocytes. (A) Left, a series of confocal images, x–y midsections, show the expression pattern for endogenous HLA-A/B/C molecules and overexpressed WT Arf6–, Arf6-Q67L–, and Arf6-T44N–EGFP constructs in nonpermeabilized CEM-CCR5 cells. Free EGFP distribution and merged images are shown. White arrows indicate Arf6-EGFP constructs and HLA-A/B/C codistribution at cell surface. Bar, 5 μm. Right, flow cytometry analysis of HLA-A/B/C cell-surface expression in Arf6-EGFP–transfected cells (control, 100% HLA-A/B/C expression in pEGFP-N1–transfected cells). Data are mean ± SEM, n = 9. (B) A series of confocal images, x–y midsections, show the expression pattern for endogenous HLA-A/B/C and overexpressed WT Arf6–, Arf6-Q67L–, and Arf6-T44N–EGFP constructs in permeabilized CEM-CCR5 cells. Free EGFP distribution and merged images are shown. Bar, 5 μm.