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. 2011 Apr 15;22(8):1148–1166. doi: 10.1091/mbc.E10-08-0722

FIGURE 4:

FIGURE 4:

Effect of different Arf6 constructs and the EFA6 factor on HIV-1 entry and infection in permissive lymphocytes. (A) Western blot analysis of endogenous Arf6, Arf6-N48I/Q67L–, and Arf6-T27N–HA expression in CEM-CCR5 cells. α-Tubulin and pcDNA 3.1 are the controls for total protein and transfected cells, respectively. A representative experiment of the three is shown. (B) Luciferase-based assay of viral entry and infection by nonreplicative X4- and R5-tropic HIV-1 viral particles, respectively, in Arf6-N48I/Q67L– and Arf6-T27N–HA–transfected CEM-CCR5 cells (control, 100% viral entry and infection in pcDNA3.1-transfected cells). Data are mean ± SEM of three independent experiments carried out in triplicate. Asterisk indicates p < 0.05, t test. (C) Western blot analysis of endogenous Arf6, WT Arf6–HA, and vsv-g-EFA6 expression in CEM-CCR5 cells. α-Tubulin and pcDNA 3.1 are the controls for total protein and transfected cells, respectively. A representative experiment of the three is shown. (D) Luciferase-based assay of viral entry and infection by nonreplicative X4- and R5-tropic HIV-1 viral particles, respectively, in WT Arf6–HA, vsv-g-EFA6, and double WT Arf6–HA/vsv-g-EFA6–transfected CEM-CCR5 cells (control, 100% viral entry and infection in pcDNA3.1-transfected cells). Data are mean ± SEM of three independent experiments carried out in triplicate.