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. 2011 Apr 15;22(8):1375–1388. doi: 10.1091/mbc.E10-06-0554

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© 2011 Yewdell et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — The Heh1p lumenal domain can rescue nup mislocalization in heh1Δpom34Δ cells. (A) Maximum intensity projections of a deconvolved z-series showing the subcellular distribution of Nup82-GFP and Nup60-GFP in heh1Δpom34Δ cells. These images are merged with a phase-contrast image in right panels. Cytoplasmic nup foci are indicated by arrows. The mislocalization of Nup60-GFP is unique, and it appears in a reticular pattern (arrowheads). The mislocalization of Nup82-GFP can be rescued by the expression of the heh2(heh1–442-735) allele (+heh1–442-735). (B) Quantitation of the percentage of cells showing nup mislocalization in the indicated strains and rescue with the heh2(heh1–442-735) allele. (C) A time-lapse series (Δt is 30 min) showing the mislocalization of Nup60-GFP in heh1Δpom34Δ cells delayed in mitosis (see Supplemental Movie S1). Left and middle panels are maximum intensity projections of a z-series of images of Nup60-GFP and Nic96-RFP, respectively, and the right panels are a merge of green, red, and phase-contrast images. Cells marked as 1, 2, or 3 are all proceeding into mitosis. By 90 min, all three cells show a redistribution of both Nup60-GFP and Nic96-RFP, most strikingly in cell 3, where there is a mitotic catastrophe that results in a loss of NE organization.