TABLE 1:
Epistatic interaction profile of HEH1 and HEH2.
| Genotypes of crosses | heh1Δ | heh2Δ | heh1Δheh2Δ | |
|---|---|---|---|---|
| Membrane ring | ||||
| pom152Δ | SL | NC | SL | |
| pom34Δ | SSS | NC | SSS* | |
| NDC1-GFP† | SS | NC | n.d. | |
| Inner ring | ||||
| nup157Δ | SSS | NC | SL | |
| nup53Δ | SS | NC | SSS | |
| nup59Δ | SS | SS | SSS | |
| nup53Δnup59Δ | SS | SS | SL | |
| nup170Δ | NC | SSS | SSS | |
| nup188Δ | NC | SL | SL | |
| Outer ring | ||||
| nup120Δ | NC | SL | SL | |
| nup84Δ | NC | SL | SL | |
| Nuclear nups and associated protein | ||||
| nup60Δ | SS | NC | SS | |
| mlp1Δ | SS | NC | SS | |
| mlp2Δ | NC | NC | NC | |
| mlp1Δmlp2Δ | SS | NC | SS | |
| nup60Δ | SS | NC | SS | |
| esc1Δ | NC | NC | NC | |
| Membrane proteins | ||||
| apq12Δ | SSS | NC | SSS* | |
| rtn1Δ | SS | NC | SS | |
| yop1Δ | NC | NC | NC | |
| rtn1Δyop1Δ | SS | NC | SS | |
| pom33Δ | NC | NC | NC | |
| per33Δ | NC | NC | NC | |
| pom33Δper33Δ | NC | NC | NC |
NC, no change in growth; n.d., not done; SL, synthetic lethality; SS, synthetic sickness; SSS, severe synthetic sickness.
*Growth of these strains was slower than that of their heh1Δ counterpart. See Supplemental Figure S2.
†Because NDC1 is essential for viability, we tested whether expression of an NDC1-GFP allele was functional in heh1Δ and heh2Δ backgrounds.