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. 2011 Apr 15;22(8):1181–1190. doi: 10.1091/mbc.E11-01-0009

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© 2011 Hongtao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The HP1–Sgo1 interaction targets Sgo1 to interphase centromeres. (A) HeLa tet-on cells that stably express Myc-Sgo1 WT or P1A were cultured in the presence of doxycycline, transfected with Sgo1 siRNA for 24 h, and stained with DAPI (blue in overlay), α-Myc (green in overlay), and CREST (red in overlay). (B) Model for the distinct binding modes and interactions of HP1 at the centromeres during interphase and mitosis. See Discussion for details.