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. 2011 Apr 15;22(8):1274–1289. doi: 10.1091/mbc.E10-08-0699

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© 2011 Helfand et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — An organized vimentin network is required for cell motility. Fibroblasts expressing the dominant-negative vimentin mutant GFP-vim lose their forward/rearward polarity (A, vim^+/+_(1–138) mEF; note GFP signal indicative of GFP-vim expression in the circularly shaped cell). These (1–138)-expressing cells exhibit continuous, circumferential ruffles but do not translocate (B, see green cell path tracing >7 h; see also Supplemental Video S5). In comparison, mEF cells not expressing the dominant-negative mutant maintained their elongated shapes and motility (B, white cell path tracings). Similarly, motility was inhibited in 3T3 cells stably expressing vimentin shRNA (C, D), in contrast to 3T3 cells stably expressing a scrambled sequence (E, F). Bars = 100 μm.