Figure 3.
Time of Intervention in HIV-1 Life Cycle. HeLa/CD4-LTR-β-gal cells were infected with HIV-1IIIB cell-free virus before A) Bacitracin (3.5 mM), B) DTNB (6 mM), C) T-20 (100 μM), D) UC781 (70 nM), E) 118-D-24 (120 μM) and F) Amprenavir (0.1 mM), were added upon HIV-1 inoculation (time zero) or at various time points post-inoculation and β-gal activity was measured following 24 hr of incubation. Percentage values are relative to the positive control (no treatment). The data represent the means ± standard deviations from three separate experiments, each of which was carried out in duplicate.