Figure 5. Staphylococcus aureus induces RANKL and inhibits OPG in osteblasts.

S. aureus (1×10⁹ cells/ml) was added to the osteoblasts for 24 hrs. Two hundred micro litres of the RIPA buffer was added to each well resulting protein was removed and centrifuged at ×10,000 g for 2 minutes. (A) RANKL or (B) OPG was detected using an ELISA kit. *P<0.001, **P<0.0001, n = 3.