Figure 2. IL-21 induces enhanced antibody secretion by CA-STAT5bER immortalized memory B cells.

Polyclonal CA-STAT5bER transduced memory B cells were deprived from tamoxifen (4HT) for 7 days and cultured with IL-2 and IL-4 or with IL-21 before IgG secretion was determined. Data shown are from one representative donor out of 5 donors. In all cultures (in culture for at least 2 months) transduced B cells had lost surface immunoglobulin expression.