Table 1. Limiting dilution culture of 100% GFP^pos TT-specific B cells.

donor	Number of positive clones from 96 wells
	Total #	from 1 c/w	from 5 c/w	from 10 c/w
A	12/96	2	10	N.D.
B	14/96	0	7	7
C	10/96	10	0	0
D	11/96	1	3	7

Indicated is the total number of clones isolated and at which cell density they were seeded. One 96 well plate was used for each condition (1, 5 or 10 cell per well). Wells contained 2500 CD40L-expressing L cells, IL-2 and IL-4. ¼ to ½ of the medium was replaced twice a week with fresh cytokines and 5000 L cells. N.D. is not determined.