Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: FEBS J. 2010 Dec 30;278(4):643–653. doi: 10.1111/j.1742-4658.2010.07985.x

Figure 2. Effects of dysfunction of the SH3 and/or SH2 domains on n-Src proteins expressed in HEK-293 cells.

Figure 2

A: Western blot showing protein expression in lysates (20 μg) of HEK-293 cells. The filters were sequentially immunoblotted with antibodies towards the proteins as indicated next to arrows. SrcpY535 (corresponding to SrcpY527): probed with anti-pY527 antibody (Rabbit); SrcpY424 (corresponding to SrcpY416): probed with anti-pY416 antibody (Rabbit). Src: probed with anti-Src antibody (Mouse). Values left of the blots indicate molecular mass in kilodaltons. B: Summary data showing kinase activity of n-Src proteins expressed in HEK-293 cells on a generic substrate (poly-Glu-Tyr); Values in brackets indicate number of experimental repeats. #: p<0.05 (Independent group t-test) in comparison with the kinase activity of constitutively active n-Src (Y535F).