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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: FEBS J. 2010 Dec 30;278(4):643–653. doi: 10.1111/j.1742-4658.2010.07985.x

Figure 4. Effects of dysfunction of the SH3 and/or SH2 domains on the phosphorylation of NMDA NR2A C-tail protein by n-Src.

Figure 4

A: Western blot showing phosphorylation of NR2A C-tail fragment proteins (aa 1096–1464, 5 μg) incubated without (“−”) or with (“+”) n-Src or its mutants as indicated. Duplicate filters were immunoblotted with antibodies towards the proteins as indicated next to arrows. NR2A: probed with anti-NR2A C-tail antibody (Rabbit); pY: probed with anti-phosphotyrosine antibody (4G10, Mouse); Src: probed with anti-Src antibody (Mouse). B: Summary data showing NR2A C-tail phosphorylation induced by n-Src proteins as indicated and detected by color assay (see Experimental Procedure). Values in brackets indicate number of experimental repeats.