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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Struct Biol. 2011 Jan 25;174(2):400–412. doi: 10.1016/j.jsb.2011.01.005

Figure 10.

Figure 10

Comparison of in-focus phase plate imaging and conventional high-underfocus imaging without phase plate. (A) Near-focus image without phase plate of a 200-nm-thick vitreously frozen cryo-ultramicrotome section of rat liver tissue. (B) Rotationally averaged power spectra of images A (IF), C (PP) and D (UF). The phase-plate cut-on spacing of 14 nm is indicated. Note improved low-frequency transfer with phase-plate imaging. (C) Near-focus image using a phase plate. (D) Image taken at 20 μm underfocus (corresponding to a spacing of 5.5 nm) without phase plate. Insets in C and D show traces across mitochondrial membranes within the white boxes on the images. Note the improved visibility of ribosomes on the endoplasmic reticulum at (*). Images recorded at 400 kV with zero-loss energy filtering. Bar = 100 nm.