Table 1. Expression of EMT and Stem-like Cell Markers, and Methylation Status of miR-200b, miR-200c, and miR-205 in Carcinogen Transformed HBECs and Lung Cancer Cell Lines.

		HBEC1MB	HBEC2MB	H1435	HCC827	Calu6	A549
EMT Marker Fold Change
	E-cadherin	-2	-659	2	0	-373	-5
	KRT19	-5	-474	2	2	-250	-5
	N-cadherin	19	237	-4	0	-10	19
	Vimentin	2	7	-7	-16	13	2
	Fibronectin	3	21	0	0	0	3
	ZEB1	3	119	-3	2	122	26
	ZEB2	109	2219	7	5	220	18
	SNAIL1	5	160	104	31	138	34
	TWIST1	6	5	-9	-2444	-930	-4
Stem Cell Markers (%)
	CD44high/CD24low	2.25	73.38	0.11	6.93	81.81	2.63
	CD133	2.96	0.69	1.00	5.88	0.74	3.62
	ALDH1	0.65	9.14	36.71	10.43	15.00	3.76
miR Fold Change (Methylation Status)
	miR-200b	-2 (M)	-55 (M)	0 (U)	0 (U)	-156 (M)	-107 (M)
	miR-200c	-2 (M)	-10 (M)	0 (U)	0 (U)	-50 (M)	-42 (M)
	miR-205	-2 (M)	-30 (M)	-55 (M)	-56 (M)	-77 (M)	-109 (M)

Gene and miRNA expression determined by qPCR. Data are mean of duplicate qPCR assays. Methylation status determined by COBRA. U, unmethylated; M, methylated.