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. Author manuscript; available in PMC: 2012 Jun 30.
Published in final edited form as: Immunol Lett. 2011 Jan 31;137(1-2):15–27. doi: 10.1016/j.imlet.2011.01.015

Figure 7. Activating mCD36 mAb enhances oxLDL-induced macrophage activation.

Figure 7

OxLDL binding to RAW-Blue cells were determined by flow cytometry (A and B). Cells were treated with mIgA, activating mCD36 mAb (JC63.1), anti-mCD36 mAb, or anti-mCD36 pAb. OxLDL binding (closed histogram) was detected using goat anti apoB IgG-biotin followed by streptavidin-PE. Cells treated identically without oxLDL (open histogram) were used as a negative control. Number indicates mean fluorescence. C, Activating mCD36 mAb enhances oxLDL-induced macrophage inflammatory response. RAW-Blue cells were co-incubated with activating mCD36 mAb (2 μg/ml) and oxLDL (5 μg/ml). Cells treated with either one of the reagents were used to determine the basal activation. SEAP activity in the supernatant was analyzed to determine kinetics of inflammatory response.