Supplementary Fig. 3.

Exogenous ChIP assay using HEK293-T cells was performed using transfected pcDNA4-PU.1 or pcDNA4-vector only. Immunoprecipitated DNA was analysed through PCR utilising primers for the CD11b promoter (previously described and thus used as a positive control) and LIMD1 promoter. +ve and −ve PCR controls were HEK293 genomic DNA and water (no DNA), respectively.