Co-localization of AJM-1::GFP and PTC-3::mRFP on the apical surface of hypodermal cells and vulval toroids in ptc-3(ok121); jcIS1 [ajm-1::gfp]; crEx341 [ptc-3::mRFP + sur-5::gfp] hermaphrodites. (A–C) AJM-1::GFP and PTC-3::mRFP fail to co-localize at pharyngeal adherens junctions. Merged Z-stack of 42 sections, each with a thickness of 0.5 μm. (D–F) Ventral view showing AJM-1::GFP and PTC-3::mRFP expression in vulval toroids and adjacent hypodermal cells. Dashed line with arrowheads indicates the direction of the ventral midline. Merged Z-stack of 8 sections, each with a thickness of 0.5 μm. (G–I) Co-localization of AJM-1::GFP and PTC-3::mRFP in posterior hypodermal cells, with posterior right. Merged Z-stack of 3 sections, each with a thickness of 0.5 μm. Arrows, hypodermal puncta co-expressing AJM-1::GFP and PTC-3::mRFP; arrowheads, nuclei expressing the SUR-5::GFP co-transformation marker. Scale bar, 10 μM.