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Western blot images of explant medium from cartilage explants alone (control, C), and treated with equine interleukin-1beta (β) (IL), or with IL-1β and a non-steroidal anti-inflammatory drug (N) combined. Blotting for cartilage intermediate layer protein-1 (CILP-1), clusterin (CLU), matrix metalloproteinase (MMP)-1 and MMP-3, thrombospondin (TSP)-1 and a pan antibody to 4 forms of TSP. Arrows indicate observed molecular weights (MW) in kilo Daltons (kDa).
