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G9a−/− mESCs show a defect in silencing of MLV provirus. (A) TT2 (WT) and G9a−/− mESCs were infected with MFG-GFP (MFG) and analyzed by flow cytometry at day 12 PI. Uninfected cells were analyzed in parallel. Data are presented as forward scatter (FSC) versus GFP. (B) Analysis of GFP expression by flow cytometry at successive time points PI. (C and D) TT2 and G9a−/− mESCs were infected with MSCV-GFP and analyzed by flow cytometry as above.
