Fig. 5.

The rate of proviral de novo DNA methylation is reduced in G9a^−/− and G9a^−/−TgC1168A cells. TT2, G9a^−/−, G9a^−/−Tg, and G9a^−/−TgC1168A mESCs were infected with MSCV-GFP. Genomic DNA from TT2 and G9a^−/− (A) or G9a^−/−Tg and G9a^−/−TgC1168A (B) mESCs was isolated at d4 and d18 PI and analyzed by bisulfite sequencing using primers specific for the proviral 5′ LTR. The presence of a methylated CpG within each sequenced molecule is indicated with a black oval, and the mean percentage of methylated CpGs/molecule sequenced is shown for each data set.