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. 2011 Mar 22;108(14):5855–5860. doi: 10.1073/pnas.1012676108

Fig. 2.

Fig. 2.

Early expression of NR2A in hippocampal slices decreases synapse number. (A) Cumulative fraction of all IEIs of mEPSCs from neurons cotransfected with NR1 and NR2A (n = 9), NR1 and NR2B (n = 8), or nontransfected age-matched neurons (black; n = 27). NR2A significantly decreases mEPSC frequency (KS test P < 0.01, D = 0.4989 vs. Ctrl and D = 0.51226 vs. NR2B). (B) Spine density from neurons cotransfected with GFP, NR1, and NR2A (n = 13), or NR2B (n = 12) and control age-matched neurons transfected with GFP (n = 8). (C) Quantification of all spine motility per micrometer from control age-matched neurons transfected with GFP (n = 8) or cells cotransfected with GFP, NR1, and NR2A (n = 13) or NR2B (n = 9). (D–F) Quantification of filopodia extension (D) and new spines per micrometer of dendrite (E), and percentage of spines retracting (F) from cells, as in C. (G) Quantification of spine volume from control neurons transfected with GFP from slices cultured for 4–5 (n = 4), 7–8 (n = 5), or 11–12 (n = 7) dic, and neurons cotransfected with GFP, NR1, and NR2A (n = 7) or NR2B (n = 4). Error bars represent SEM.