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. 2011 Mar 22;108(14):5855–5860. doi: 10.1073/pnas.1012676108

Fig. 4.

Fig. 4.

Knockdown of NR2B in hippocampal slices decreases synapse number and reduces spine motility. (A) Cumulative fraction of all IEIs of AMPAR-mediated mEPSCs from neurons transfected with 2B-siRNA (n = 8; KS D = 0.4876 vs. Ctrl), 2A-siRNA (n = 11; KS D = 0.1176 vs. Ctrl), or scrambled siRNA (dashed line; n = 11) and age-matched control neurons (black; n = 4 cells). (B) Quantification of dendritic spine density in control neurons (n = 8), scrambled siRNA (n = 6), and neurons cotransfected with GFP and NR2B-siRNA (n = 10) or NR2A-siRNA (n = 6). (C) Quantification of all spine motility events per micrometer from control neurons transfected with GFP (n = 8), scrambled siRNA (n = 6), 2B-siRNA (n = 8), or 2A-siRNA (n = 6). (D–F) Quantification of filopodia extension (D) and new spines per micrometer of dendrite (E), and percentage of spines retracting (F) from cells, as in C. Error bars represent SEM.