. 2011 Apr;178(4):1701–1714. doi: 10.1016/j.ajpath.2010.12.053

Table 1.

Cellular Composition of Blood and Spleen of Wild-Type and Homozygous meB2 Littermates Assessed by Flow Cytometry

Cell type^†	Peripheral blood				Spleen
	Healthy		Ptpn6^meB2/meB2		Healthy		Ptpn6^meB2/meB2
	% total	% sub	% total	% sub	% total	% sub	% total	% sub
T cell (CD3)	51.2 ± 3.2		22.2 ± 1.4^⁎		47.8 ± 3.6		22.1 ± 0.3^⁎
CD8⁺ T cell	17.2 ± 4.1		15.6 ± 3.4		6.8 ± 0.5		4.4 ± 0.1
CD4⁺ T cell^†	30.0 ± 0.8		11.0 ± 2.5^⁎		26.7 ± 0.6		17.1 ± 0.1^⁎
Subpopulation
CD4⁺CD69⁺	0.3 ± 0.1	1.0	1.2 ± 0.1^⁎	10.9^⁎⁎	6.2 ± 1.0	23.2	9.1 ± 2.3^⁎	53.2^⁎⁎
CD4⁺CD44^high	15.9 ± 1.5	53.0	9.5 ± 2.2^⁎	86.4^⁎⁎	12.4 ± 1.0	46.4	5.8 ± 0.3^⁎⁎	33.9

B cell (B220⁺)	27.3 ± 0.8		16.3 ± 1.0^⁎		26.3 ± 4.7		14.2 ± 0.9^⁎⁎
B cell (B220⁺)	27.3 ± 0.8		16.3 ± 1.0^⁎		26.3 ± 4.7		14.2 ± 0.9^⁎⁎
Subpopulation
B1a/b	6.9 ± 1.1	25.3	7.2 ± 3.9	44.3^⁎⁎	6.1 ± 1.6	23.2	8.5 ± 2.4	60.5^⁎⁎
B2 (conventional)	17.3 ± 1.6	63.4	15.7 ± 5.4	52.3^⁎	16.1 ± 2.5	61.3	4.1 ± 2.1	28.8^⁎⁎
CD69⁺B220⁺	0.3 ± 0.2	1.1	0.2 ± 0.1	1.5	0.8 ± 1.2	2.2	1.1 ± 0.2	7.8^⁎

Gr-1⁺ neutrophils	36.2 ± 2.3		78.8 ± 2.3^⁎⁎		27.0 ± 4.0		79.6 ± 2.8^⁎⁎
Subpopulation^‡
Live	17.7 ± 4.3	66.5	63.4 ± 4.4	80.5^⁎	8.8 ± 1.3	51.8	48.2 ± 4.3	60.6
Pre-apoptotic	7.4 ± 0.3	27.8	12.4 ± 2.4	15.7^⁎⁎	2.9 ± 0.3	17.1	27.3 ± 5.6	34.9^⁎⁎
Late apoptotic	2.5 ± 0.3	9.4	4.1 ± 0.9	5.2^⁎⁎	5.3 ± 0.4	31.2	4.6 ± 1.2	5.8^⁎⁎

Cells were analyzed (in all cases, n = 8 sick and n = 7 healthy littermates) at 2 days after disease onset together with their wild-type littermates.

Data are reported as percentage of the mean ± SEM.

AnxV, annexin V; PI, propidium iodide; sub, subpopulation.

^⁎

P < 0.05;

^⁎⁎

P < 0.01 compared with healthy mice.

^†

Because of the relative lymphopenia, T-cell and B-cell numbers may be similar in healthy and sick (Ptpn6^meB2/meB2) mice, but the ratio (activated cell types, or B1a/b cells) is significantly higher in the sick mice.

^‡

Live cells: AnxV⁻PI⁻. Pre-apoptotic cells: AnxV⁺PI⁻. Late apoptotic: AnxV⁺PI⁺.