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The tumor vessel area is increased in mouse B16-BL6 melanoma during pregnancy. Quantitative analyses were performed after staining of CD31- for blood vessels and Lyve-1 for lymphatic vessels. By using automated morphometric analyses, the following parameters were compared: vascular density, size of vessels, and ratio of relative area occupied by vessels. Results are expressed as mean ± SE. The Student's t-test was used. *P < 0.05, **P < 0.01, and ***P < 0.001.
