Table 2.
Expression of Genes Involved in the Leptin Signaling Pathway, Adaptive Thermogenesis, and Fatty Acid Metabolism in TG and Control Mice
| Tissue | Genes | TG mice | Wild-type mice | P value |
|---|---|---|---|---|
| Liver | AMPK | 1.12 ± 0.23 (n = 6) | 1.98 ± 0.23 (n = 4) | <0.0001 |
| CPT1 | 1.14 ± 0.19 (n = 6) | 2.26 ± 0.26 (n = 4) | 0.0005 | |
| ACCβ | 1.84 ± 0.18 (n = 6) | 1.0 ± 0.08 (n = 4) | <0.0001 | |
| Kidney | AMPK | 1.0 ± 0.17 (n = 3) | 1.34 ± 0.24 (n = 4) | 0.087 |
| CPT1 | 0.94 ± 0.1 (n = 4) | 1.35 ± 0.24 (n = 4) | 0.025 | |
| ACCβ | 1.6 ± 0.23 (n = 4) | 1.07 ± 0.16 (n = 4) | 0.01 | |
| Brown fat | Leptin | 2.57 ± 0.41 (n = 6) | 0.92 ± 0.15 (n = 4) | 0.002 |
| UCP1 | 0.64 ± 0.09 (n = 4) | 3.0 ± 0.15 (n = 3) | <0.0001 | |
| UCP2 | 1.0 ± 0.21 (n = 4) | 11.0 ± 2.18 (n = 3) | 0.014 | |
| AMPK | 0.27 ± 0.03 (n = 5) | 0.43 ± 0.06 (n = 4) | 0.0034 | |
| White fat | Leptin | 3.75 ± 0.47 (n = 5) | 1.07 ± 0.24 (n = 5) | <0.001 |
| Lepr-b | 1.1 ± 0.29 (n = 4) | 1.14 ± 0.15 (n = 4) | NS | |
| SOCS-3 | 0.95 ± 0.11 (n = 4) | 0.98 ± 0.33 (n = 4) | NS | |
| Hypothalamus | Lepr-b | 1.01 ± 0.23 (n = 4) | 1.03 ± 0.18 (n = 4) | NS |
| SOCS-3 | 1.24 ± 0.29 (n = 4) | 0.91 ± 0.17 (n = 4) | NS | |
| AGRP | 1.96 ± 0.28 (n = 4) | 1.29 ± 0.18 (n = 4) | <0.001 | |
| POMC | 0.5 ± 0.09 (n = 4) | 0.74 ± 11 (n = 4) | <0.001 | |
| Leptin | 3.99 ± 1.4 (n = 4) | 0.57 ± 21 (n = 4) | <0.001 | |
| MC4R | 1.0 ± 0.29 (n = 4) | 1.1 ± 0.26 (n = 4) | NS | |
| Skeletal muscle | AMPK | 1.05 ± 0.22 (n = 5) | 1.86 ± 0.13 (n = 4) | 0.001 |
| CPT1 | 1.35 ± 0.22 (n = 3) | 3.63 ± 0.46 (n = 3) | 0.001 | |
| UCP2 | 2.0 ± 0.27 (n = 3) | 2.1 ± 0.50 (n = 3) | NS | |
| UCP3 | 1.66 ± 0.47 (n = 3) | 4.11 ± 1.7 (n = 5) | 0.034 |
The mRNA levels were normalized against endogenous mouse β-actin and expressed as the mean ± SE (n = 3 to 6 mice); all mice were male, aged 11 months, and from the same TG founder.
ACC, acetyl CoA carboxylase; Lepr-b, leptin receptor; NS, statistically not significant (P > 0.05); SOCS, suppressor of cytokine signaling.