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. 2011 Apr;178(4):1847–1860. doi: 10.1016/j.ajpath.2010.12.046

Figure 5.

Figure 5

Characterization of CANT1 expression in prostatic cell lines. Cells were cultivated and treated as described below, and CANT1 mRNA and protein expression was determined by QRT-PCR (top) and Western blot analysis (bottom), respectively. A: LNCaP, DU-145, PC-3, and RWPE-1 cells were cultivated in T75 cell culture flasks until a density of 70% to 80%. A total of 300,000 LNCaP (B) and 200,000 PC-3 cells (C) were seeded into 6-well plates, transfected with siRNAs as indicated, and incubated for 72 hours.