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Proliferation of prostate cancer cell lines on CANT1 knockdown. A total of 300,000 LNCaP (A) and 200,000 PC-3 cells (B) were seeded in 6-well plates, transfected with siRNAs as indicated, and counted after 72 hours. A total of 600,000 LNCaP (C) and 400,000 PC-3 cells (D) were seeded in 6-cm dishes and transfected with siRNAs as indicated. After 72 hours, 20,000 LNCaP and 10,000 PC-3 cells were reseeded in quadruplicates into 96-well plates, and DNA synthesis rate was measured.
