Table 1.
Antibodies Used in Immunohistochemistry, Immunofluorescence, and Western Blot
| Primary antibody | Commercial source | Catalog no. | Species | Antibody type | Working concentration |
|---|---|---|---|---|---|
| Caveolin-1 (N-20) | Santa Cruz Biotechnology | sc-894 | Rabbit | Polyclonal | IF 1:50 |
| WB 1:1000 | |||||
| Caveolin-1 | Upstate | 05-762 | Mouse | Monoclonal, IgG2b | IF 1:50 |
| Caveolin-1 | BD | 610407 | Mouse | Monoclonal, IgG1 | IF 1:50 |
| NeuN | Chemicon | MAB377B | Mouse | Monoclonal, IgG1 | IF 1:200 |
| CD31 | BD | 553370 | Rat | Monoclonal, IgG2a, κ | IF 1:50 |
| GFAP | Zymed | 13-0300 | Rat | Monoclonal, IgG2a, κ | IF 1:400 |
| Iba1 | Wako | 016-20001 | Rabbit | Polyclonal | IF 1:1000 |
| HO-1 | Stressgen | SPA-895 | Rabbit | Polyclonal | IF 1:400 |
| WB 1:1000 | |||||
| NTi | Upstate | 06-284 | Rabbit | Polyclonal | IHC 1:200 |
| MPO | Dako | A0398 | Rabbit | Polyclonal | IHC 1:1000 |
| COX-2 | Cayman | 160106 | Rabbit | Polyclonal | WB 1:1000 |
| α-tubulin | Santa Cruz Biotechnology | SC-23948 | Mouse | Monoclonal, IgG1 | WB 1:5000 |
IF, immunofluorescence; WB, Western blotting; IgG2b, immunoglobulin G2b; NeuN, neuronal nuclei antigen (neuron marker); CD31, endothelial cell marker; GFAP, glial fibrillary acidic protein (astrocyte marker); Iba1, ionized calcium binding adaptor molecule 1 (microglia marker); HO-1, heme-oxygenase-1; NT, nitrotyrosine, peroxynitrite (ONOO−) marker; IHC, immunohistochemistry; MPO, myeloperoxidase (neutrophil marker); COX-2, cyclooxygenase-2.