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. 2011 Apr;178(4):1556–1567. doi: 10.1016/j.ajpath.2010.12.025

Figure 3.

Figure 3

Inhibition of TGFβ1-induced myofibroblast differentiation by ITE is not specific to orbital fibroblasts, and it results in decreased myofibroblast activity, as demonstrated by collagen gel contraction assay. A: Human corneal fibroblasts were treated with TGFβ1 (1 ng/mL) and ITE (1 μmol/L) for 1, 3, or 5 days. Cell lysates were Western blotted using antibodies specific for α-SMA and GAPDH. B: Human lung fibroblasts were treated with TGFβ1 (1 ng/mL) and ITE (0 to 20 μmol/L) for 96 hours. Cell lysates were Western blotted using antibodies specific for fibronectin, α-SMA, calponin, CYP1B1, and GAPDH. C: Collagen gels were prepared with primary human orbital fibroblasts and treated with TGFβ1 (1 ng/mL) and ITE (1 μmol/L) for 4 days. Collagen gels were weighed and percent contraction, as compared with gels without cells, is shown. *P < 0.001 compared with TGFβ1 alone (Student's t-test).