FIG. 7.

Effect of 2-aminoethoxydiphenyl borate (2-APB) on H₂O₂-induced glutamate-independent injury of mouse cortical neurons. (a) Addition of 30 μM 2-APB, a concentration known to completely inhibit the transient receptor potential melastatin 2 (TRPM2) current but has small effect on the TRPM7 current, did not provide protection against H₂O₂-induced injury (n = 8 wells each). (b) Addition of 100 μM 2-APB, a concentration known to inhibit the TRPM7 channels, significantly reduced H₂O₂-induced neuronal injury (n = 15–16, ^**p < 0.01 compared to 100 μM H₂O₂ group). (c) Neuronal injury induced by higher concentration of H₂O₂ (500 μM) was not protected by 100 μM 2-APB (n = 11–12).