FIG. 1.
An example of a fission event that yields an ∼9 mV difference between the two daughter mitochondria. The pseudocolor represents the membrane potential and is derived from a ratio image of TMRE and matrix-targeted photoactivatable (mtPA) GFP. The pseudocolored image depicts two time spots, 60 s before fission and 160 s after fission. Scale bar: 2 μm. Charts of mitochondrial membrane potential (Δψm) over time under control conditions are shown to the right. Numbers (i)–(iii) depict the three most common changes to membrane during a fission events as observed in INS1 cells. (see text for further details). Data are modified from Twig et al. (110). (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars).