Table 1.
Effect of Manipulations in Mitochondrial Proteins Opa1, Mfn1/2, Drp1, and Fis1 on Autophagy and Mitophagy
| Manipulation | Cell type | Effect on mitophagy | Reference | Comments |
|---|---|---|---|---|
| Fis1 RNAi | INS1 β-cells | Reduction of 70% in APs containing mitochondria | (110) | No change in the mass of APs containing ER |
| Fis1 Overexpression | HeLa cells, INS1 β-cells | Reduces total mitochondrial volume by ∼50% | (28, 83) | No change in ER morphology or total ER area |
| HeLa cells | 50% increase in total APs mass. No direct assessment of mitophagy | (30) | Qualitative analysis suggests medium-high correlation between LC3 and mitochondrial markers | |
| Drp1K38A | INS1 β-cells | Reduction of 75% in APs containing mitochondria | (110) | No change in the mass of APs containing ER |
| Drp1 RNAi | HeLa cells | Decrease in mitophagy (qualitative) | (85) | A general increase in autophagy. |
| Drp1 overexpression | HeLa cells | 70% decrease in mitochondrial mass | (4) | No specific tests for mitophagy |
| Opa1 Overexpression | INS1 β-cells | Reduction of 63% in APs containing mitochondria | (110) | No change in the mass of APs containing ER |
| Opa1 heterozygous mutant mice | Retinal Ganglion cells | An increase in general autophagy | (120) | No specific tests for mitophagy |
| Mfn1 overexpression | INS1 β-cells | 40% decrease in mitochondrial volume | (83) | No specific tests for mitophagy |
| Mfn1 loss of function (overexpression of Mfn1 DN) | INS1 β-cells | Unchanged mitochondrial mass | (83) | Insignificant slight decrease in mitochondrial volume. No specific tests for mitophagy. |
| Mfn2 KO | MEFs | Inhibition of autophagy | (36) | No specific tests for mitophagy |
Note that in some works mitophagy was assessed directly by colocalization between mitochondrial and AP markers, indirectly as changes in mitochondrial mass, or not distinguished from autophagy.
AP, autophagosome; ER, endoplasmic reticulum; KO, knockout.