Figure 3.

NRG1 has no significant effect on excitatory synapse development in cortical glutamatergic neurons. NRG1 (5 nm) was applied to the low-density DIV12 dissociated cortical neurons for 2 d. GABA, CaMKII, and PSD-95 were stained to represent GABAergic interneurons, glutamatergic neurons, and excitatory synapses, respectively. PSD-95 staining on GABA-negative or CaMKII-positive neurons without or with NRG1 treatment is shown in A and D, respectively. Scale bars: 30 μm; boxed area was enlarged in the respective bottom image. B, C, E, F, PSD-95 puncta, scored as in Figure 1. Data shown are the mean ± SEM of three individual experiments. For GABA-negative neurons (n = 59 and 65 for control and treated groups, respectively), puncta numbers are 13.1 ± 0.73 and 11.6 ± 0.67 and sizes are 15.8 ± 1.46 and 17.1 ± 2.31 in the absence and presence of NRG1, respectively. For CaMKII-positive neurons (n = 74 and 72 for control and treated groups, respectively), puncta numbers are 11.8 ± 0.58 and 12.1 ± 0.61 and sizes are 15.3 ± 0.75 and 15.3 ± 0.88 in the absence and presence of NRG1, respectively.