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Temperature dependence of Kd and ΔmP values. Cbl(TKB) (9 nM – 20 μM) was titrated against 100 nM ZAP70 peptide 1. The Kd and ΔmP values were obtained through a nonlinear least squares fit to a single-site binding model. For each temperature, values were measured after 30 min incubations.
