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Effect of Cbl(TKB) concentration on assay parameters. Displacement of labeled ZAP70 peptide 1 from His-Cbl(TKB) by unlabeled ZAP70 peptide 2 in varying concentrations of His-Cbl(TKB). (IC50 @9 μM = 13.0 ± 1.7 μM, IC50 @5 μM = 7.5 ± 1.6 μM, IC50 @2.5 μM = 5.4 ± 1.6 μM, Ki(average) = 0.5 ± 0.2 μM).
