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. 2011 Mar 15;104(8):1303–1312. doi: 10.1038/bjc.2011.53

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Copyright © 2011 Cancer Research UK

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mRNA expression of inhibin genes in disease-free (N), and tumour-adjacent (NT) liver and in HCC (T). (A) Real-time PCR cycle threshold (C_t) values normalised to RNA input are given. C_t values for B2M are shown for comparison. Boxes (blank, N; dotted, NT; grey; T) represent the lower and upper quartiles with medians; whiskers illustrate the 10–90 percentiles of the samples. Significant changes (P<0.05) are marked with brackets and asterisk. (B and C) Expression changes of individual NT or T samples related to the median of normal liver (N). Log transformed C_t values were used. Dotted lines indicate expression changes to >2-fold or <0.5-fold, considered as thresholds of biological relevance. INHA levels in three N and one NT samples were undetectable and for calculations were set at the detection limit (C_t 40).