Skip to main content
. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Nature. 2011 Mar 13;472(7342):230–233. doi: 10.1038/nature09932

Table 1.

Equilibrium dissociation constants for the binding of AXPs to phosphorylated AMPK.

Ligand Kd (μM) Kd,I (μM) Kd,II (μM)
vs NADH vs C-AXPs
AMP 1.6 (0.5) 2.5 (0.6) 80 (25)
ADP 1.3 (0.5) 1.5 (0.4) 50 (15)
ATP 0.9 (0.3) 1.7 (0.5) 65 (15)
Mg-ATP 32 (12) 18 (7.5) 230 (80)

Dissociation constants were determined at 20°C by competition against NADH or C-AXPs in 25 mM Tris, 1 mM TCEP, 100 mM NaCl (pH 8) with and without 5 mM MgCl2. The Kd values are reported as the mean (± SD) determined from at least three independent measurements.