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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Nature. 2011 Mar 13;471(7339):532–536. doi: 10.1038/nature09772

Figure 2. RPA displacement by hnRNPA1.

Figure 2

a, ssTEL and ssMUT precoated with RPA were incubated with NEs. After the incubation, the proteins bound to DNA were retrieved, eluted, and applied to RPA-coated ssTEL or ssMUT (see Supplemental Methods). After the second incubation, the remaining RPA2 on DNA was analyzed by Western blot. b, Proteins captured by RPA-ssTEL or RPA-ssMUT and eluted by salt were analyzed by Western blot using antibodies to hnRNPA1, hnRNPA2/B1, and TRF2. c, RPA-coated ssTEL (0.8 nM) was incubated with increasing concentrations of WCEs (0.06, 0.24, 0.96 μg/μl). The hnRNPA1 and hnRNPA2/B1 bound to DNA and the remaining RPA2 on DNA were analyzed by Western blot. d, RPA-coated ssTEL or ssMUT (0.8 nM) was incubated with increasing concentrations of purified hnRNPA1 (2.4, 4.8, 7.2 nM). The remaining RPA2 on DNA was analyzed as in a. e, POT1-coated ssTEL (0.8 nM) was incubated with increasing concentrations of purified hnRNPA1 (2.4, 4.8, 7.2 nM).

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