Table 1.
Primer sequences used in guinea pig arcuate scRT-PCR
| Gene name | Accession number or scaffold number | Product size (bp) | Primer sequence | Primer position |
|---|---|---|---|---|
| Kiss1 | 1321987 | 67 | GTGTCACCTCCTTGGGAGAAC | Forward 7-27 |
| TGGCCTGTGGGTCTAGGAT | Reverse 73-55 | |||
| Dyn | U38912 | 184 | AAGTGCGAGAGGGATGGTGTG | Forward 147-167 |
| CTCAGAGGGCAGCAAGGTTTC | Reverse 330-310 | |||
| POMC | S78260 | 206 | CTGGCCTTGCTGCTTCAGAT | Forward 40-59 |
| AAGTGGCCCGTGACGTACTT | Reverse 245-226 | |||
| NPY | M15789 | 126 | CTGCGACACTACATCAACC | Forward 168-186 |
| GTCTTCAAGCCGAGTTCTG | Reverse 293-275 | |||
| LRb | scaffold_2.549.1 | 124 | ATGCTGGGCGCACTGTTAATAG | Forward 2947-2968 |
| GCTCAAATGTTTCGGGCTTCTG | Reverse 3070-3049 | |||
| TRPC1 | scaffold_8.413.1 | 167 | TGCCCAAGCCCGGAATTCAC | Forward 1256-1275 |
| GCTGGCAGTTAGACTGGGACAC | Reverse 1422-1401 | |||
| TRPC4 | DQ008965 | 136 | GGTTAAGCTGCAAAGGCATACG | Forward 1580-1601 |
| CCATGCTGTGCTTTCACATTGG | Reverse 1715-1694 | |||
| TRPC5 | DQ008966 | 167 | GGCTTCTCAGCACATTGTC | Forward 1131-1149 |
| CCATCAGGTTCCACCAATC | Reverse 1297-1279 | |||
| PLCγ1 | scaffold_45.086.1 | 226 | GATGAAGAGGAGCCCAAAG | Forward 1307-1325 |
| CATTTCGCCAGAAGGACAG | Reverse 1532-1514 |